Biolegend, 308804, PE anti-human CD210 (IL-10 R) Antibody, 100tests

CD210, also known as the IL-10 receptor, is a 90-110 kD protein expressed on T cells, B cells, NK cells, monocytes and macrophages. CD210 belongs to the class II cytokine receptor family which includes the IFN-γ receptor (CDw119), the IFN-α/β receptor (CD118) and tissue factor (CD142). The IL-10 receptor is involved in signal transduction by inducing phosphorylation of STAT1a and STAT3 and by inducing activation of Jak1 and Tyk.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: shIL-10R
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking1-3 of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).
Application References(PubMed link indicates BioLegend citation): Liu Y, et al. 1997. J. Immunol. 158:604. (Immunogen, IP, Block) Levings MK, et al. 2005. Blood 105:1162. (Block) Goodier MR, et al. 2000. J. Immunol. 165:139. (Block) Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Liu BS,et al. 2011. J Leukoc Biol. 89:981. PubMed Joffe M, et al. 2012. Int Immunol. 24:447. PubMed MacDonald KP, et al. 1999. J. Immunol. 163:5599. (epitope)
Product Citations: Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed Ni G, et al. 2019. PLoS One. 14:e0213813. PubMed Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed Vahl J, et al. 2017. Br J Cancer.. 10.1038/bjc.2017.336. PubMed Barry J, et al. 2016. Sci Rep. 6:21244. PubMed Eriksson E, et al. 2019. J Immunol. 202:787. PubMed Sun Z, et al. 2015. Cancer Res. 75:1635. PubMed
RRID: AB_314735 (BioLegend Cat. No. 308803) AB_314736 (BioLegend Cat. No. 308804)
Structure: Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
Distribution: T cells and B cells, NK cells, monocytes, macrophages
Function: Activates STAT1, STAT3, Jak1, Tyk
Ligand/Receptor: IL-10
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223. 2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.
Gene ID: 3587
UniProt: View information about CD210 on UniProt.org
Clone: 3F9
Regulatory Status: RUO
Workshop: VII 70502
Other Names: IL-10 Receptor, IL-10R
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.