Biolegend, 309223, APC anti-human CD2 Antibody, 25tests

CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of T cell activation, and partial blocking of B cell costimulation2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 309235 & 309236).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds.1995. Leucocyte Typing V Oxford University Press. New York. Hughes CCW, et al. 1996. J. Biol. Chem. 271:5369.
Product Citations: Lameris R, et al. 2023. Cell Rep Med. 100961:4. PubMed Nakagawa R, et al. 2024. Nat Commun. 10516:15. PubMed Xu X, et al. 2021. Molecular Cell. :. PubMed
RRID: AB_2687218 (BioLegend Cat. No. 309223) AB_2687219 (BioLegend Cat. No. 309224)
Structure: Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution: T cells, NK cells, thymocytes, and thymic B cell subsets
Function: T cell activation, adhesion
Ligand/Receptor: CD58 (LFA-3), CD48, CD59, CD15
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Bell G, et al. 1995. J. Immunol. 155:2805. 2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.
Gene ID: 914
UniProt: View information about CD2 on UniProt.org
Clone: TS1/8
Regulatory Status: RUO
Workshop: V S025
Other Names: LFA-2, T11, SRBC-R
Isotype: Mouse IgG1, κ