Biolegend, 309804, PE anti-human CD137 (4-1BB) Antibody, 100tests

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ectodomain of recombinant human 4-1BB fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP) Salih HR, et al. 2000. J. Immunol. 165:2903. (FA) Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA) Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations: Liu T, et al. 2021. Gut. 70:1965. PubMed Chow A 2023. Immunity. 56(1):93-106.e6. PubMed Shitaoka K, et al. 2018. Cancer Immunol Res. 6:378. PubMed Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed Goncharov MM, et al. 2022. Elife. 11:. PubMed Shin JJ, et al. 2022. J Clin Immunol. :. PubMed Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed Pavlicek D, et al. 2017. Immun Ageing. 14:22. PubMed Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed Elias G, et al. 2022. Elife. 11:. PubMed Correnti CE, et al. 2020. Nat Struct Mol Biol. 27:342. PubMed Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed Senger K, et al. 2022. Curr Protoc. 2:e538. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID: AB_314782 (BioLegend Cat. No. 309803) AB_314783 (BioLegend Cat. No. 309804)
Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand
Cell Type: T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gruss H, et al. 1995. Blood 85:3378. 2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355. 3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219. 4. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: 4B4-1
Regulatory Status: RUO
Workshop: VI C-7
Other Names: 4-1BB, ILA, CD137, TNFRSF9
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.