Biolegend, 310307, Purified anti-human CD135 (Flt-3/Flk-2) Antibody, 100μg

CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL, and CML-BC. CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD135 (Flt-3/Flk-2) recombinant protein fragment
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W22045B does not affect the binding of clones W22045C and BV10A4H2 on target cells.
RRID: AB_3698965 (BioLegend Cat. No. 310307)
Structure: Member of the immunoglobulin supergene family, type 3 tyrosine kinase receptor; 130-160 kD
Distribution: Expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. It is also expressed on AML, ALL, and CML-BC tumor cells.
Function: Tyrosine kinase growth factor receptor involved in the growth and differentiation of primitive hematopoietic cells
Ligand/Receptor: FLT3 ligand
Cell Type: B cells, Hematopoietic stem and progenitors, Leukemia, Thymocytes
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Rappold I, et al. 1997. Blood. 90:111-25. Rosnet O, et al. 1996. Acta Haematol. 95:218-23. Rosnet O, et al. 1996. Leukemia. 10:238-48. Bertho JM, et al. 2000. Scand J Immunol. 52:53-61.
Gene ID: 2322
UniProt: View information about CD135 on UniProt.org
Clone: W22045B
Regulatory Status: RUO
Other Names: FMS-like tyrosine kinase-3, FLT-3, STK-1, Flk-2
Isotype: Rat IgG2a, κ