Biolegend, 310604, Spark Violet™ 423 anti-human IL-2 Antibody, 100tests

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human IL-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3068183 (BioLegend Cat. No. 310603) AB_3068183 (BioLegend Cat. No. 310604)
Structure: Cytokine; 15.4 kD (Mammalian)
Distribution: T cells
Function: Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Ligand/Receptor: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Taniguchi T, et al. 1993. Cell 73:5. 3. Nistico G. 1993. Prog. Neurobiol. 40:463. 4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Gene ID: 3558
UniProt: View information about IL-2 on UniProt.org
Clone: W19046A
Regulatory Status: RUO
Other Names: Interleukin-2, T cell growth factor (TCGF), Killer cell helper factor (KHF); Macrophage-activating factor for cytotoxicity I (MAF-C I)
Isotype: Rat IgG2b, κ