Biolegend, 310805, PE anti-human CD154 (CD40L) Antibody, 25tests

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF) Rushworth SA, et al. 2002. Transplantation 73:635. (Block) Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF) Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block) Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed Kuchen S, et al. 2007. J. Immunol. 179:5886. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Wang Y, et al. 2023. Hum Vaccin Immunother. 19:2202127. PubMed Yu ED, et al. 2022. Cell Rep Methods. 2:100350. PubMed Lim JME 2023. STAR Protocols. 4(1):101995. PubMed Pandit H, et al. 2023. iScience. 26:105929. PubMed Babl N, et al. 2023. Front Oncol. 13:1107484. PubMed Collora JA, et al. 2022. Immunity. 55:1013. PubMed Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed Karnell J, et al. 2011. J Immunol. 187:3603. PubMed Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed Kuchen S, et al. 2007. J Immunol. 179:5886. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed Zhang J, et al. 2020. Nat Microbiol. . PubMed Bziat V, et al. 2021. Cell. . PubMed Yang W, et al. 2019. Nat Med. 25:767. PubMed Shifrut E et al. 2018. Cell. 175(7):1958-1971 . PubMed Biondi BE, et al. 2021. Contemp Clin Trials Commun. 21:100704. PubMed Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
RRID: AB_314828 (BioLegend Cat. No. 310805) AB_314829 (BioLegend Cat. No. 310806)
Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227. 2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275. 3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959
UniProt: View information about CD154 on UniProt.org
Clone: 24-31
Regulatory Status: RUO
Other Names: CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.