Biolegend, 311203, Purified anti-human CD227 (MUC-1) Antibody, 100μg

Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2/neu. Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2/neu.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Membrane enriched fractions of human metastatic breast carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone DF3 recognizes the extracellular domain of the MUC1 protein, specifically the tandem repeat region.
Application References(PubMed link indicates BioLegend citation): Hamada T, et al.2004. J Clin Pathol. 57:813-21 Hamada T, et al. 2012. Cancer. 118:5251-64. Yasui S, et al. 1994. Acta dermato-venereologica. 74:98-100.
RRID: AB_3698967 (BioLegend Cat. No. 311203)
Structure: 500-1000 kD proteoglycan (glycosylated form)
Distribution: Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers
Interaction: Complexes with SOS1 and Grb2 in RAS signaling
Ligand/Receptor: ICAM-1 and Her2/neu (ErbB2)
Cell Type: Dendritic cells, Epithelial cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Yasui S, et al. 1994. Acta Derm Venereol. 74:98-100. Ichige K, et al. 1995. Clin Cancer Res. 1:565-71.
Gene ID: 4582
UniProt: View information about CD227 on UniProt.org
Clone: DF3
Regulatory Status: RUO
Other Names: Mucin 1, Episialin, MAM-6, CA 15-3, PEM, CD227, EMA
Isotype: Mouse IgG1, κ