Biolegend, 313707, PE anti-human CD195 (CCR5) Antibody, 25tests

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Recent studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with human CCR5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1Clone HEK/1/85a and clone HEK/1/85a/7a are the same.
Application References(PubMed link indicates BioLegend citation): Mueller A, et al. 2002. Blood 99:785. Al-Odi E, et al. 2012. PLoS One. 7:e42217. PubMed Osman A, et al. 2014. J Immunol. 192:5083. PubMed Liu S, et al. 2014. Virus Res. 192:92. PubMed
Product Citations: Tian L, et al. 2022. Nat Cancer. 3:1318. PubMed Sarra M, et al. 2011. J Immunol. 186:5435. PubMed Hu J, et al. 2013. Cytokine. 62:141. PubMed Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed Gonzalo–Gil E, et al. 2019. Elife. 8:e44360. PubMed Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed Matsuda K, et al. 2015. Biochem Biophys Res Commun. 457:288. PubMed
RRID: AB_345307 (BioLegend Cat. No. 313707)
Structure: G-coupled receptor family 1, membrane protein, 45 kD
Distribution: Subset of T cells, monocytes
Function: Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1
Ligand/Receptor: MIP-1α, MIP-1ß, RANTES
Cell Type: Dendritic cells, Monocytes, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661. 5. Lai J, et al. 2003. CVI. 10:1123. 6. Mañes S, et al. 2003. J. Exp. Med. 198:1381. 7. Vaday GG, et al. 2006. Prostate 66:124.
Gene ID: 1234
UniProt: View information about CD195 on UniProt.org
Clone: HEK/1/85a
Regulatory Status: RUO
Other Names: CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.