Biolegend, 315203, FITC anti-human CD43 Antibody, 25tests

CD43 is a 95-135 kD type I transmembrane glycoprotein known as leukosialin, leukocyte sialoglycoprotein, sialophorin, and galactoglyprotein. It is expressed at high levels on the surface of all leukocytes (except on resting B cells) and at low levels on platelets, negative on erythrocytes. The expression of CD43 on T cells and neutrophils is rapidly downregulated by proteolytic shedding after activation with various stimuli. The soluble form of CD43 is present in human serum. It has been reported that CD43 plays a role in anti-adhesive functions, but also mediates adhesion via its interaction with CD54 or some lectins. Similarly, CD43 is implicated in anti-apoptotic and proapoptotic signaling.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human T lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemistry of formalin-fixed paraffin-embedded sections, and T cell activationSup>1,2.
Application References(PubMed link indicates BioLegend citation): Olweus J, et al. 1993. J. Immunol. 151:707.Alvarado M, et al. 1995. Eur. J. Immunol. 25:1051.Ye L, et al. 2013. Stem Cell Transl Med. 2:558. PubMed
Product Citations: Ye L, et al. 2013. Stem Cells Trans Med. 2:558. PubMed Xie X, et al. 2024. iScience. 110445:27. PubMed Qu K, et al. 2024. iScience. 110409:27. PubMed Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
RRID: AB_389246 (BioLegend Cat. No. 315203) AB_389247 (BioLegend Cat. No. 315204)
Structure: Type I membrane protein, 95-135 kD, soluble form exists in serum
Distribution: Thymocytes, T cells, neutrophils, plasma cells, myelomas
Function: Anti-adhesion, mediate adhesion, regulate T cell activation and neutrophil migration, antiapoptotic and proapoptotic signaling
Interaction: HIPK2
Ligand/Receptor: CD54, Counterreceptor for SN/Siglec-1
Modification: Highly glycosylated
Cell Type: Thymocytes, T cells, Neutrophils, Plasma cells, B cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Leukocyte Typing IV. Knapp W, et al. (Eds.) Oxford University Press (1989) 2. Stephanova I, et al. 1989. Mol. Immunol. 26:153. 3. Alvarado M, et al. 1995. Eur. J. Immunol. 25:1051. 4. Deckert M, et al. 1992. Eur. J. Immunol. 22:2943 5. Cermak L, et al. 2002. J. Biol. Chem. 227:7955.
Gene ID: 6693
UniProt: View information about CD43 on UniProt.org
Clone: MEM-59
Regulatory Status: RUO
Other Names: Leukosialin, leuokocyte sialoglycoprotein, sialophorin, galactoglyprotein
Isotype: Mouse IgG1, κ