Biolegend, 316311, APC anti-human β2-microglobulin Antibody, 25tests

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8 + T cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human β2-microglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.
Application References(PubMed link indicates BioLegend citation): Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
Product Citations: Chung H et al. 2018. Cell. 172(4):811-824 . PubMed Chimienti R, et al. 2022. Cell Rep. 40:111423. PubMed Sim MJW, et al. 2022. Elife. 11: . PubMed Le TA, et al. 2022. Mol Ther Nucleic Acids. 30:621. PubMed Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed Zhuang X, et al. 2020. Front Immunol. 2.415972222. PubMed Gondim M, et al. 2015. J Virol. 89: 12518 - 12524. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Kishton RJ, et al. 2022. Cell Rep. 40:111153. PubMed DeWeirdt PC, et al. 2020. Nat Biotechnol. . PubMed Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed Haideri T, et al. 2022. Cell Rep Methods. 2:100290. PubMed Xu X, et al. 2018. Sci Rep. 8:11649. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed
RRID: AB_10643412 (BioLegend Cat. No. 316311) AB_10641281 (BioLegend Cat. No. 316312)
Structure: Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Distribution: Leukocytes, platelets, endothelial cells, epithelial cells
Function: Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
Ligand/Receptor: CD3/TCR complex, CD8
Cell Type: Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13. 2. Williams DB, et al. 1989. J. Immunol. 142:2796. 3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533. 4. Williams A, et al. 2002. Tissue Antigens 59:3.
Gene ID: 567
UniProt: View information about beta2-microglobulin on UniProt.org
Clone: 2M2
Regulatory Status: RUO
Other Names: β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype: Mouse IgG1, κ