Biolegend, 316505, FITC anti-human Ig light chain κ Antibody, 25tests

The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
Product Citations: Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed
RRID: AB_493611 (BioLegend Cat. No. 316506)
Structure: Ig family
Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3514
UniProt: View information about Ig light chain kappa on UniProt.org
Clone: MHK-49
Regulatory Status: RUO
Other Names: Immunoglobulin light chain kappa
Isotype: Mouse IgG1, κ