Biolegend, 317410, PE anti-human CD4 Antibody, 100tests

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human peripheral T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations: Ping X, et al. 2022. DNA Repair (Amst). 119:103394. PubMed Masuda H, et al. 2022. MAbs. 14:2122275. PubMed Yi X, et al. 2023. J Med Virol. 95:e28642. PubMed Claeys E, et al. 2021. Front Immunol. 12:650731. PubMed Asashima H, et al. 2022. J Clin Invest. 132: . PubMed Tunali G, et al. 2023. J Clin Invest. :. PubMed Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed Li Z, et al. 2019. PLoS Pathog. 15:e1007498. PubMed Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed Estes J, et al. 2015. J Infect Dis. 211:744. PubMed Bhargava R, et al. 2020. J Biol Chem. 295:125. PubMed Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed Cisneros-Aguirre M, et al. 2022. Nat Commun. 13:3662. PubMed Wang H, et al. 2022. iScience. 25:105065. PubMed Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed Chang HH, et al. 2019. J Autoimmun. 101:121. PubMed Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Sitlinger A, et al. 2021. Cells. 10:. PubMed Mozhgani SH, et al. 2019. Retrovirology. 16:46. PubMed Sievers C, et al. 2012. Clin Immunol. 143:162. PubMed Dawson NA, et al. 2019. JCI Insight. 4:6. PubMed Landgraf KE, et al. 2020. Commun Biol. 0.330555556. PubMed Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed Bhargava R, et al. 2020. J Biol Chem. 295:125. PubMed Zimmerman KA, et al. 2019. Physiol Rep. 7:e13951. PubMed Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
RRID: AB_571954 (BioLegend Cat. No. 317409) AB_571955 (BioLegend Cat. No. 317410)
Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: OKT4
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T4
Isotype: Mouse IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.