Biolegend, 318302, Purified anti-human CD56 (NCAM) Antibody, 100μg

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Gunesch JT, et al. 2020. Elife. 9: . PubMed van der Poel M, et al. 2020. J Immunol. 205:2511. PubMed Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed Weinhage T, et al. 2020. J Immunol. 205:56. PubMed Manickam C, et al. 2018. AIDS. 32:1571. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Ardain A, et al. 2019. Nature. 570:528. PubMed Crome SQ, et al. 2017. Nat Med. 1.213888889. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Sung JA, et al. 2018. Mol Ther. 26:2496. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID: AB_604092 (BioLegend Cat. No. 318302)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ