Biolegend, 318321, PerCP/Cyanine5.5 anti-human CD56 (NCAM) Antibody, 25tests

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The CD56 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Vielle NJ, et al. 2018. Sci Rep. 8:5440. PubMed Shen Q, et al. 2022. Cancer Res Commun. 2:258. PubMed Shemesh A, et al. 2022. J Exp Med. 219: . PubMed Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed Walk J, et al. 2019. Nat Commun. 10:874. PubMed Yokoyama K, et al. 2022. Front Immunol. 13:1016914. PubMed Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed Ciccia F, et al. 2012. Ann Rheum Dis. 71:295. PubMed Shemesh A, et al. 2022. J Exp Med. 219:. PubMed McDonnell A, et al. 2017. BMC Cancer . 10.1186/s12885-017-3403-5. PubMed Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID: AB_893391 (BioLegend Cat. No. 318321) AB_893389 (BioLegend Cat. No. 318322)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.