Biolegend, 318334, Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody, 100tests

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed Kim MY, et al. 2021. JCI Insight. 6:. PubMed Zhang T, et al. 2022. Am J Reprod Immunol. 87:e13524. PubMed Macedo AB, et al. 2022. J Virol. 96:e0037222. PubMed McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed Kritikou J, et al. 2016. Sci Rep. 6:30636. PubMed Bigley A, et al. 2013. Brain Behav Immun. 889:1591. PubMed Klichinsky M, et al. 2020. Nat Biotechnol. 38:947. PubMed Sorensen ES, et al. 2020. Antimicrob Agents Chemother. 64:00:00. PubMed Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed Kip P, et al. 2021. Nutrients. 13:. PubMed Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed Jackson E, et al. 2017. PLoS One. 10.1371/journal.pone.0185160. PubMed Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Du J, et al. 2014. Cancer Immunol Res. 2:878. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed Kinloch NN, et al. 2021. Nat Commun. 12:165. PubMed Jansen D, et al. 2015. Rheumatology. 54:728. PubMed Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed Hernndez DC, et al. 2021. Immunity. :. PubMed Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed Garfall AL, et al. 2018. JCI Insight. 3. PubMed Kroloff M, et al. 2022. J Immunother Cancer. 10:. PubMed Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID: AB_11142683 (BioLegend Cat. No. 318333) AB_2561912 (BioLegend Cat. No. 318334)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ