Biolegend, 320805, PE anti-human CD314 (NKG2D) Antibody, 25tests

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8 + T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Wu J, et al. 1999. Science 285:730. Wu J, et al. 2000. J. Exp. Med. 192:1059. Groh V, et al. 2001. Nature Immunol. 2:255. Wu J, et al. 2002. J. Immunol. 169:1236. Roberts A, et al. 2001. J. Immunol. 167:5527. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Watanbe M, et al. 2014. Int Immunol. PubMed
Product Citations: Firouzi J, et al. 2023. Cell J. 25:92. PubMed Dorsch M, et al. 2020. Eur J Immunol. 50:656. PubMed Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Lee Y, et al. 2020. J Innate Immun. 1:. PubMed Liu R, et al. 2022. Aging Dis. 13:1576. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Myers JA, et al. 2022. JCI Insight. :. PubMed Whitman E, Barber A 2015. Mol Immunol. 63:268. PubMed Zhou J, et al. 2015. J Immunol. 194:4688. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Karampatzakis A, et al. 2021. Front Immunol. 12:641521. PubMed Mohan S, et al. 2009. Haematologica. 94:1407. PubMed ça T, et al. 2010. Blood. 115:2407. PubMed Sweeney EE, et al. 2020. Nano Res. 13:736. PubMed Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed Wolpert F, et al. 2015. PLoS One. 10: e0139603. PubMed Hejazi M, et al. 2022. Front Immunol. 12:798087. PubMed Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed Chu P, et al. 2015. PLoS One. 10:125664. PubMed Sharma N,et al. 2017. J Immunol. 10.4049/jimmunol.1700647. PubMed Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed Vidard L, et al. 2019. J Immunol. 203:676. PubMed Li M, et al. 2020. Virol Sin. 35:588. PubMed Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed Chu P, et al. 2017. PLoS One. 12(6):e0179096. PubMed
RRID: AB_492961 (BioLegend Cat. No. 320805) AB_492960 (BioLegend Cat. No. 320806)
Structure: C-type lectin
Distribution: NK cells, γ/δ T cells, CD8+ T cells
Function: Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor: MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Jamieson AM, et al. 2002. Immunity 17:19. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667. 7. Maasho K, et al. 2005. J. Immunol. 174:4480. 8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Gene ID: 22914
UniProt: View information about CD314 on UniProt.org
Clone: 1D11
Regulatory Status: RUO
Other Names: NKG2D
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.