Biolegend, 321102, Purified anti-human CD206 (MMR) Antibody, 100μg

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human mannose receptor
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).
Application References(PubMed link indicates BioLegend citation): Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block) Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block) Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations: Gallucci L, et al. 2023. EMBO Rep. 24:e56818. PubMed Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Behrens NE, et al. 2019. MBio. 10:2. PubMed Laudanski K, et al. 2018. J Transl Med. 0.765972222. PubMed Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed Muir LA, et al. 2022. JCI Insight. 7:. PubMed Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed Azari S, et al. 2021. MBio. 12:e0184921. PubMed Hu G, et al. 2021. Nat Commun. 12:773. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Gardet A, et al. 2010. J Immunol. 185:5577. PubMed Brasil da Costa FH, et al. 2020. PLoS One. 15:e0230354. PubMed Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed Lee SE, et al. 2021. JCI Insight. 6:. PubMed Li S, et al. 2019. Front Neurol. 1.036805556. PubMed De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed Cao H, et al. 2021. Nat Commun. 12:1792. PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed Trumpi K, et al. 2018. Oncotarget. 3. PubMed Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID: AB_571922 (BioLegend Cat. No. 321101) AB_571923 (BioLegend Cat. No. 321102)
Structure: Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution: Macrophages, dendritic cells, hepatic and lymphatic endothelial cells
Function: Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor: Mannose, fucose, N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303 2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469. 4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934. 5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.
Gene ID: 4360
UniProt: View information about CD206 on UniProt.org
Clone: 15-2
Regulatory Status: RUO
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype: Mouse IgG1, κ