Biolegend, 321204, PE anti-human/mouse Integrin β7 Antibody, 100tests

Integrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,α IEL /β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.
100tests
Verified Reactivity: Human, Mouse, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Chimpanzee
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).
Application References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block) Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed Durant L, et al. 2020. Microbiome. 8:88. PubMed Candia E, et al. 2017. PLoS One. 10.1371/journal.pone.0182009. PubMed Min HK, et al. 2021. Immune Netw. 21:e43. PubMed Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID: AB_571970 (BioLegend Cat. No. 321203) AB_571971 (BioLegend Cat. No. 321204)
Structure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution: CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
Function: Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor: CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Picarella D, et al. 1997. J. Immunol. 158:2099. 3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635 4. Cepek KL, et al. 1994. Nautre 372:190.
Gene ID: 164213695
UniProt: View information about Integrin beta7 on UniProt.org
Clone: FIB504
Regulatory Status: RUO
Workshop: VI 6T-101, VI A024
Other Names: β7 Integrin, integrin βp, ITGB7
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.