Biolegend, 321507, Purified anti-human FcRn Antibody, 100μg

FcRn is a 43 kD transmembrane MHC class I-like glycoprotein. It forms the neonatal Fc receptor through a non-covalent association with β2-microglobulin. FcRn is expressed in the vascular endothelium, blood-brain barrier, kidney, and several leukocyte subsets, including monocytes, neutrophils, and dendritic cells. In association with β2-microglobulin, FcRn binds to human IgG, and transports maternal antibodies to the fetus antenatally across the placenta. In adults, FcRn plays a crucial role in extending the half-lives of IgG and albumin by recycling and preventing them from degradation. Additionally, FcRn can promote antigen presentation by enhancing IgG-mediated phagocytosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human FcRn transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested FC, Block - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For surface flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone S23035A can block the binding of human IgG to cell surface FcRn. Clone S23035A can detect FcRn on cell surface and intracellularly.
RRID: AB_3720493 (BioLegend Cat. No. 321507)
Structure: Transmembrane MHC class I-like glycoprotein, 43 kD
Distribution: Monocytes, neutrophils, and dendritic cells
Function: Transport IgG across cell membrane, recycling IgG and extending its half-life in the circulation
Ligand/Receptor: Human IgG
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Adaptive Immunity, Cell Biology, Immunology
Molecular Family: Fc Receptors
Antigen References: Story CM, et al. 1994. J Exp Med. 2377-81. Kandil E, et al. 1996. Cytogenet Cell Genet. 73: 97–8.
Gene ID: 2217
UniProt: View information about FcRn on UniProt.org
Clone: S23035A
Regulatory Status: RUO
Other Names: IgG Fc fragment receptor transporter alpha chain, FcgammaRn alpha-chain, Neonatal Fc receptor
Isotype: Mouse IgG1, κ