Biolegend, 322906, PE anti-human TSLPR (TSLP-R) Antibody, 100tests

TSLP-R, also known as thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind TSLP. The TSLP-R contains a WSXWS motif required for proper protein folding and a box1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight approximately 40 kD, and two isoforms have been reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells including dendritic cells and activated monocytes, and is weakly expressed in T cells. Expression has also been reported in heart, skeletal muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3 and STAT5 to stimulate cell proliferation. Ligand receptor interactions haves been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergic diseases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TSLP-R:Fc protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 322905/322906) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Bouchlaka MN, et al. 2013. J Exp Med. 210:2223. PubMed
Product Citations: Nováková M, et al. 2020. Haematologica. . PubMed Bouchlaka M, et al. 2013. J Exp Med. 210:2223. PubMed
RRID: AB_2085328 (BioLegend Cat. No. 322906)
Structure: Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs. Predicted molecular weight approximately 4
Distribution: Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal muscle, and kidney
Function: TSLP binding to functional receptor activates JAK2, STAT3 and STAT5 to stimulate cell proliferation. Has been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T
Interaction: Together with IL-7R binds TSLP
Cell Type: Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Reche PA,et al. 2001. J. Immunol. 167:336. 2. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23. 3. Zhang W, et al. 2001. Biochem. Biophys. Res. Commun. 281:878. 4. Wang YH, et al. 2006. Immunity 24:827.
Gene ID: 64109
UniProt: View information about TSLPR on UniProt.org
Clone: 1D3
Regulatory Status: RUO
Other Names: Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype: Mouse IgG2a, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.