Biolegend, 324302, Purified anti-human CD334 (FGFR4) Antibody, 100μg

The 4FR6D3 antibody recognizes human CD334, also known as fibroblast growth factor 4, FGFR4 and TKF. CD334 is a member of the fibroblast growth factor receptor family and a cell surface tyrosine kinase containing three immunoglobulin-like domains. FGFR4 has a predicted molecular weight of approximately 88 kD and is widely expressed in many tissues including the intestine, muscle, heart, cornea, retina, and pancreas, with highest expression in lung and kidney.CD334 binds acidic fibroblast growth factors (FGF8, GFG1, FGF19, FGF6, and FGF2); ligand binding induces mitogenesis and differentiation. CD334 has been shown to interact with STAT1 and STAT3 and is over-expressed in gynecological tumors suggesting a possible role for this receptor in breast and ovarian tumorigenesis. Unique alleles (Arg388) in FGFR4 have been shown to be associated with cancer progression and increased tumor cell motility. The 4FR6D3 antibody has been shown to be useful for flow cytometry.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH-3T3 cells transfected with full-length human FGFR4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Bussolati B, et al. 2012. Am J Physiol Renal Physiol. 302:116. PubMed.
Product Citations: Bussolati B, et al. 2012. Am J Physiol Renal Physiol. 302:F116. PubMed
RRID: AB_756088 (BioLegend Cat. No. 324302)
Structure: Member of the fibroblast growth factor receptor family. Cell surface tyrosine kinase containing three immunoglobulin-like domains. Predicted molecular weight approximately 88 kD.
Distribution: Widely expressed in many tissues including the intestine, muscle, heart, cornea, retina, and pancreas; highest expression in lung and kidney
Function: Ligand binding induces mitogenesis and differentiation; over-expressed in gynecological tumors suggesting a possible role for this receptor in breast and ovarian tumorigenesis. Unique alleles (Arg388) in FGFR4 have been associated with cancer progression
Interaction: STAT1, STAT3
Ligand/Receptor: Binds acidic fibroblast growth factors (FGF8, GFG1, FGF19, FGF6, and FGF2)
Cell Type: Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Holtrich U, et al. 1991. Proc. Natl. Acad. Sci. USA 88:10411. 2. Partanen J, et al. 1991. EMBO J. 10:1347. 3. Bange J, et al. 2002. Cancer Res. 62:840. 4. Vainikka S, et al. 1992. EMBO J. 11:4273.
Gene ID: 2264
UniProt: View information about CD334 on UniProt.org
Clone: 4FR6D3
Regulatory Status: RUO
Other Names: FGFR4, fibroblast growth factor receptor 4, TKF, FGF R4
Isotype: Mouse IgG1, κ