Biolegend, 325705, APC anti-human CD53 Recombinant Antibody, 25tests

CD53 is a 35-42 kD type III tetraspan membrane protein. It is expressed on all leukocytes, but not on platelets or erythrocytes. CD53 is thought to be involved in signal transduction. It has been shown to interact with a number of proteins including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highly glycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein by approximately 25 kD. The HI29 antibody has been shown to be useful for flow cytometry, and immunohistochemistry (frozen).
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2894566 (BioLegend Cat. No. 325705) AB_2894566 (BioLegend Cat. No. 325706)
Structure: Cell surface protein, tetraspan family, four membrane spanning hydrophobic domains, 35-42 kD.
Distribution: Hematopoietic cells including neutrophils, monocytes, B cells, T cells (single positive thymocytes and peripheral T cells), and eosinophils. Not expressed on platelets, erythrocytes, and non-hematopoietic cells.
Function: Signal transduction, cross-linking on B cells causes activation.
Interaction: IL-4, CD20, CD2, CD9, IL-2, integrin α4, integrin β1, CD4
Ligand/Receptor: NK cell, B cells, Neutrophils, Eosinophils
Antigen References: Amiot M. 1990. J Immunol. 145:4322-5. Angelisova P, et al. 1990. Immunogenetics. 32:281-5. Olweus J, et al. 1993. J Immunol. 153:4997. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford: Oxford University Press.
Gene ID: 963
UniProt: View information about CD53 on UniProt.org
Clone: QA19A07
Regulatory Status: RUO
Other Names: TSPAN25
Isotype: Mouse IgG1, κ