Biolegend, 327101, Purified anti-human myeloid antigen Antibody, 25μg

BM-1 antigen is a 183 kD myeloid-specific-DNA-binding protein which is expressed in myeloid cells, including myeloid precursors and mature granulocytes. It is also expressed in chronic myeloid leukemia (CML) and M2 and M3 acute myeloid leukemias (AML) as well as myelomonocytic leukemias, but not in T, B, NK and other non-hematopoietic cells. The biological function of this antigen is not clear, although it has been proposed that BM-1 may play a role in the differentiation of myeloid cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PWM-stimulated human PBL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality testedIHC-P, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemical staining of frozen and paraffin sections1, and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Epstein AL, et al.1987. Blood 70:1124.
RRID: AB_893592 (BioLegend Cat. No. 327102)
Structure: 183 kD, DNA-binding protein
Distribution: In myeloid cells
Antigen References: 1. Epstein AL, et al. 1987. Blood 70:1124.
Gene ID: 861
UniProt: View information about myeloid antigen on UniProt.org
Clone: BM-1
Regulatory Status: RUO
Other Names: myeloid specific antigen, p183 DNA-binding cytoplasmic protein
Isotype: Mouse IgG1, κ