Biolegend, 328120, Alexa Fluor® 700 anti-human CD90 (Thy1) Antibody, 100μg

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34 + cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34 + CD90 + cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HEL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Application References(PubMed link indicates BioLegend citation): Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
Product Citations: Madhu V, et al. 2014. PLoS One. e103060:9. PubMed James JL, et al. 2021. Cartilage. 13:544S. PubMed Lina IA, et al. 2022. Otolaryngol Head Neck Surg. 166:712. PubMed Mian S, et al. 2015. Nat Commun. 6: 10004. PubMed Marquez-Curtis LA, et al. 2022. PLoS One. 17:e0263005. PubMed Amoah A, et al. 2021. Haematologica. Online ahead of print. PubMed Jung J, et al. 2019. Cell Rep. 26:1906. PubMed Felker S, et al. 2022. JCI Insight. 7:. PubMed Li Y, et al. 2020. Front Cell Dev Biol. 8:604021. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Henson F, et al. 2021. J Orthop Res. 39:1411. PubMed Niehage C, et al. 2011. PLoS One. 6:e20399. PubMed
RRID: AB_2203303 (BioLegend Cat. No. 328119) AB_2203302 (BioLegend Cat. No. 328120)
Structure: 25-35 kD glycoprotein, Ig superfamily
Distribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
Function: Regulate hematopoiesis and neural cell growth, cell adhesion
Ligand/Receptor: β3 integrin, β2 integrin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850.
Gene ID: 7070
UniProt: View information about CD90 on UniProt.org
Clone: 5E10
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ