Biolegend, 328205, FITC anti-human CD39 Antibody, 25tests

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory like T cells. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PHA activated human lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3, immunofluorescence4, immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Aversa GG, et al. 1988. Transplant. P. 20:4952. Aversa GG, et al. 1989. Transplant. P. 21:34950. Borsellino G, et al. 2007. Blood. 110:1225. (Block) Stockl J, et al. 2001. J. Immunol. 167:2724. (IF) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Langhans B, et al. 2012. PLoS One. 7:e42094. PubMed Balachandran H, et al. 2022. Cell Rep. 38:110345. PubMed Hsiao CC, et al. 2023. iScience. 26:105785. PubMed Labanieh L, et al. 2022. Cell. 185:1745. PubMed Kochetkova I, et al. 2014. J Immunol. 192:804. PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed Yerneni SS, et al. 2021. MBio. 12:e0165721. PubMed Chen P, et al. 2021. Front Immunol. 12:689019. PubMed Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed Fang F, et al. 2022. JCI Insight. 7:. PubMed Kudryavtsev I, et al. 2019. Front Genet. 10:604. PubMed Van Zeebroeck L, et al. 2021. Front Immunol. 12:655122. PubMed Ludwig N, et al. 2020. Angiogenesis. 1.374305556. PubMed Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed Kim SP, et al. 2022. Cancer Immunol Res. :OF1. PubMed Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
RRID: AB_940423 (BioLegend Cat. No. 328205) AB_940425 (BioLegend Cat. No. 328206)
Distribution: Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.
Cell Type: B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 953
UniProt: View information about CD39 on UniProt.org
Clone: A1
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: gp80, E-ATPDase, NTPDase-1, ecto-apyrase, Ec3.6.1.5
Isotype: Mouse IgG1, κ