Biolegend, 328402, Purified anti-human CD100 Antibody, 100μg

CD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
100μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PHA activated lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Hall K, et al. 1996. P. Natl. Acad. Sci. USA 93:11780. Mizrahi S, et al. 2007. PLoS One. 2(9):e818. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
RRID: AB_1236386 (BioLegend Cat. No. 328401)
Structure: Class IV semaphorin family member, 150 kD homodimer
Distribution: T cells, B cells, weak on NK cells
Function: Costimulatory molecule in adaptive immune responses that switches off negative signaling from CD72
Cell Type: B cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman SL Bloumsell W Gilks et al. eds. 1995. Leucocyte Typing V:White Cell Differentiation Antigens. Oxford University Press New York. 2. Bougeret CIG Mansur H Dastot et al. 1992. Increased surface expression of a newly identified 150 kDa dimer early after
Gene ID: 10507
UniProt: View information about CD100 on UniProt.org
Clone: A8
Regulatory Status: RUO
Workshop: V BP383, S233
Other Names: Sema4D
Isotype: Mouse IgG1, κ