Biolegend, 329630, KIRAVIA Blue 520™ anti-human CD273 (B7-DC, PD-L2) Antibody, 100tests

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PD-L2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Brown JA, et al. 2003. J. Immunol. 170:1257 Rodig N, et al. 2003. Eur. J. Immunol. 33:3117
RRID: AB_2922553 (BioLegend Cat. No. 329629) AB_2922553 (BioLegend Cat. No. 329630)
Structure: B7 Immunoglobulin superfamily, 25 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor: PD-1
Cell Type: Dendritic cells, Macrophages
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Shin T, et al. 2003. J. Exp. Med. 198:31. 2. Liu X, et al. 2003. J. Exp. Med. 197:1721. 3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
Gene ID: 80380
UniProt: View information about CD273 on UniProt.org
Clone: 24F.10C12
Regulatory Status: RUO
Other Names: B7-DC, PD-L2, PDL2, B7DC
Isotype: Mouse IgG2a, κ