Biolegend, 331406, FITC anti-human TCR Vδ2 Antibody, 100tests

The Vδ2 TCR is a variant of the TCR δ chain expressed on a subset of γ/δ T cells. Vγ9Vδ2 T lymphocytes, a major γ/δ T cell subset in humans, recognize phosphoantigens, certain tumor cells, and cells treated with aminobisphosphonates. This cell population displays cytolytic activity against various tumor cells. The γ/δ TCR is an heterodimeric TCR complex composed of covalently bound γ and δ chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Rojas RE, et al. 2005. J. Infect. Dis. 192:1806. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Jeon SB, et al. 2023. Cell Prolif. 56:e13366. PubMed Zarobkiewicz M, et al. 2022. J Clin Med. 11: . PubMed Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed Garrido C, et al. 2018. JCI Insight. 3:e120121. PubMed Vyborova A, et al. 2022. Front Immunol. 13:915366. PubMed Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Wang Z, et al. 2018. Front Immunol. 9:1239. PubMed Tsai C, et al. 2015. J Immunol. 194:3890. PubMed
RRID: AB_1089231 (BioLegend Cat. No. 331405) AB_1089230 (BioLegend Cat. No. 331406)
Structure: Ig superfamily member, TCR γ/δ with CD3 forms the CD3/TCR complex
Distribution: Expressed on majority of peripheral γ/δT cells
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound to MHC
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Scotet E, et al. 2005. Immunity 22:71. 2. Rincon-Orozco B, et al. 2005. J. Immunol. 175:2144.
Gene ID: 6964
UniProt: View information about TCR Vdelta2 on UniProt.org
Clone: B6
Regulatory Status: RUO
Other Names: T cell receptor V δ 2, TRDV2, TCRDV102S1
Isotype: Mouse IgG1, κ