Biolegend, 331601, Purified anti-human CD276 (B7-H3) Antibody, 25μg

B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with mouse B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Dong C, et al. 2024. Front Immunol. 1295693:15. PubMed
RRID: AB_1279202 (BioLegend Cat. No. 331602)
Structure: About 49.7 KD transmembrane protein characterized by extracellular IgV and IgC domains.
Distribution: B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.
Ligand/Receptor: The receptor has not been identified, but it seems to be expressed on activated T cells.
Bioactivity: B7-H3 is a negative regulator of T cell activation and IL-2 production.
Cell Type: Antigen-presenting cells, B cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Sun M, et al. 2002. J. Immunology. 168:6294 2. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235
Gene ID: 80381
UniProt: View information about CD276 on UniProt.org
Clone: DCN.70
Regulatory Status: RUO
Other Names: B7RP2
Isotype: Mouse IgG1, κ