Biolegend, 333402, Purified anti-human CD35 Antibody, 100μg

CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Hogg N, et al. 1984. Eur. J. Immunol. 14:236 Zabeleta J, et al. 1998. Clin Diag. Lab. Immunol. 5:690 Pascual M, et. al. 1994. Eur. J. Immunol. 24:702 Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Weinhage T, et al. 2020. J Immunol. 205:56. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
RRID: AB_1089032 (BioLegend Cat. No. 333402)
Structure: Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD
Distribution: Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells
Function: Adhesion, mediate phagocytosis, inhibit T-cell proliferation
Ligand/Receptor: C3b, iC3, C4b, iC4
Cell Type: B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS 2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699
Gene ID: 1378
UniProt: View information about CD35 on UniProt.org
Clone: E11
Regulatory Status: RUO
Workshop: III 204
Other Names: C3b/C4b receptor, complement receptor type 1 (CR1)
Isotype: Mouse IgG1, κ