Biolegend, 334207, Alexa Fluor® 488 anti-human CD202b (Tie2/Tek) Antibody, 25tests

CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant extracellular domain of human Tie2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA
Application References(PubMed link indicates BioLegend citation): Peters KG, et al. 1998. Br. J. Cancer. 77:51. Wong AL, et al. 1997. Circ. Res. 81:567. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.
Product Citations: Jonasch E, et al. 2011. Ann Oncol. 2.764583333. PubMed
RRID: AB_1186028 (BioLegend Cat. No. 334208)
Structure: 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution: Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
Function: Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand/Receptor: Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1.De Palma M, et al. 2005. Cancer Cell. 8:211 2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168 3. Hsu HC, et al. 2000. Blood. 96:3757 4. Arai F, et al. 2004. Cell. 118:149
Gene ID: 7010
UniProt: View information about CD202b on UniProt.org
Clone: 33.1 (Ab33)
Regulatory Status: RUO
Other Names: Tie2, TEK
Isotype: Mouse IgG1, κ