Biolegend, 334310, APC anti-human CD40 Antibody, 100tests

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pound JD, et al. 1999. Int. Immunol. 11:11. Shey MS, et al. 2014. J Immunol. 192:4833. PubMed Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
Product Citations: Kalus P, et al. 2022. Int J Mol Sci. 23:. PubMed Rodriguez-Perdigon M, et al. 2023. Front Bioeng Biotechnol. 11:1159819. PubMed Thwe PM et al. 2017. Cell metabolism. 26(3):558-567 . PubMed Casasola-LaMacchia A, et al. 2021. Sci Rep. 11:1028. PubMed Yi H, Lu G 2012. Cell Immunol. 277:44. PubMed Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID: AB_2075792 (BioLegend Cat. No. 334309) AB_2260153 (BioLegend Cat. No. 334310)
Structure: TNFR superfamily, type I glycoprotein, 48 kD
Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor: CD154 (CD40 ligand)
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881. 2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
Gene ID: 958
UniProt: View information about CD40 on UniProt.org
Clone: 5C3
Regulatory Status: RUO
Workshop: V CD40.4
Other Names: BP50, TNFRSF5
Isotype: Mouse IgG1, κ