Biolegend, 334908, Purified anti-human CD158z (KIR3DL3) Antibody, 100μg

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIMs and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158z is a glycoprotein with three extracellular Ig domains and a long cytoplasmic tail containing an ITIM motif. It is reportedly expressed on intraepithelial γδ and CD8+ T cells. KIR3DL3 has been reported to bind to HHLA2 (B7H7) to cause downstream functional inhibition.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KIR3DL3-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Based on in-house testing on different KIR-expressing transfectants, clone S23011E does not interact with other KIR protein family members.
RRID: AB_3675052 (BioLegend Cat. No. 334908)
Structure: Three Ig-like extracellular domain glycoprotein
Distribution: Intraepithelial γδ and CD8+ T cells
Function: T Cell subset regulation
Ligand/Receptor: HHLA2
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Vilches C, et al. 2002. Annu. Rev. Immunol. 92.107361. Middleton D, et al. 2010. Immunol. 123:8. Bhatt RS, et al. 2021. Cancer Immunol. 2:156-169.
Gene ID: 115653
UniProt: View information about CD158z on UniProt.org
Clone: S23011E
Regulatory Status: RUO
Other Names: CD158z, Killer Cell Immunoglobulin-Like Receptor 3DL3, KIR44, KIRC1
Isotype: Mouse IgG2a, κ