Biolegend, 335005, APC anti-human CD158h (KIR2DS1) Antibody, 25tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIMs and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158h is a 50 kD, single-pass glycoprotein with two extracellular Ig domains that contains a short cytoplasmic tail and does not contain an intracellular ITIM domain. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR2DS1 is reported to bind certain HLA-C antigens on target cells (HLA-C*02, HLA-C*04, HLAC*06, for example).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KIR2DS1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Based on in-house testing, clone S22019F does not react to other KIR protein family members.
RRID: AB_3717093 (BioLegend Cat. No. 335005) AB_3717093 (BioLegend Cat. No. 335006)
Structure: 50 kD two Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, small subset of T Cells
Function: NK cell regulation
Ligand/Receptor: Some HLA-C molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Biassoni, R. et al. 1996. J Exp Med. 183:645-50. Stewart CA, et al. 2005. Proc Natl Acad Sci. 102:13224-9. Lanier, LL. 2005. Annu Rev Immunol. 23:225-74.
Gene ID: 3806
UniProt: View information about CD158h on UniProt.org
Clone: S22019F
Regulatory Status: RUO
Other Names: CD158H, Killer Cell Immunoglobulin-Like Receptor 2DS1, KIR2DS1
Isotype: Mouse IgG1, κ