Biolegend, 336230, Brilliant Violet 421™ anti-human CD36 Antibody, 100tests

CD36 is an 85 kD integral membrane glycoprotein, also known as GPIIIb, or GPIV. It is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, macrophages/monocytes and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor, binding thrombospondin, long chain fatty acids, oxidized LDL, collagen type I, IV, and V as well as apoptotic cells. The 5-271 antibody has been reported to be useful for flow cytometry.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human platelets
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence2.
Application References(PubMed link indicates BioLegend citation): Stelner E, et al. 2006. J. Cell Sci. 119:459. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations: Wen Z, et al. 2023. Adv Healthc Mater. e2300970:12. PubMed Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed Xu S, et al. 2021. Immunity. . PubMed
RRID: AB_2814227 (BioLegend Cat. No. 336229) AB_2814228 (BioLegend Cat. No. 336230)
Structure: Integral membrane protein, CD36 family, single chain glycoprotein, 85-113 kD
Distribution: Expressed in various epithelial and endothelial cells, erythrocytes, platelets, monocytes/ macrophages, some macrophage-derived dendritic cells
Function: Scavenger receptor for thrombospondin, long chain fatty acids, anionic phospholipids, oxidized LDL, collagen types I, IV and V. Involved in recognition and phagocytosis of apoptotic cells, cell adhesion.
Ligand/Receptor: Thrombospondin, anionic phospholipids, oxidized LDL, collage types I, IV, and V
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Erythrocytes, Macrophages, Monocytes, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Hogg N, et al. 1984. Immunology 53-753. 2. Greenwalt DE, et al. 1992. Blood 80:1105. 3. Armsesilla AL, et al.1994. J. Biol. Chem. 269:18985. 4. Endemann G, et al. 1993. J. Biol. Chem. 268:11811.
Gene ID: 948
UniProt: View information about CD36 on UniProt.org
Clone: 5-271
Regulatory Status: RUO
Other Names: GPIIIb, GPIV
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.