Biolegend, 337303, FITC anti-human CD172a/b (SIRPα/β) Antibody, 25tests

SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that belong to Ig superfamily. SIRPa (CD172a, SIRPb),(SIRPb1,CD172b), and SIRPg (SIRPb2,CD172g) have been described. CD172a/b molecules are expressed on myeloid cells and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration. CD47 (IAP) is an extracellular ligand for CD172a. CD172b is a disulfide-linked homodimer. CD172b has been shown to regulate neutrophil transepithelial migration through the interaction with DAP12 and Syk kinase (through TYROBP).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: mAb 148 is specific for CD172a/b, and also stains CD172g transfected cells, but not T-lymphocytes
Application References(PubMed link indicates BioLegend citation): Piccio L, et al. 2005. Blood 105:2421 Dietrich J, et al. 2000. J. Immunol. 164:9
Product Citations: Connor SM, et al. 2022. Front Cell Neurosci. 16:894601. PubMed
RRID: AB_2254620 (BioLegend Cat. No. 337304)
Structure: Signal-regulatory-protein family, Ig superfamily
Distribution: Monocytes/macrophages, granulocytes, dendritic cells, neurons
Ligand/Receptor: CD172a binds CD47, CD172b interacts with DAP12
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kharitonenkov A, et al. 1997. Nature 386:181. 2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147. 3. Seiffert M, et al. 2001. Blood 97:2741. 4. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838. 5. Barclay AN and MH. Brown. 2006. Nat. Rev.Immnuol. 6:457
Gene ID: 10326140885
UniProt: View information about CD172a/b on UniProt.org
Clone: 148
Regulatory Status: RUO
Other Names: SIRPa, SIRPb, SIRPalpha/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B
Isotype: Mouse IgG1, κ