Biolegend, 337904, PE anti-human CD85h (ILT1) Antibody, 100tests

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subset of ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while the inhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85h is a 69kD activating receptor, also known as ILT1, LIR-7, or LILRA2. ILT1 is found on the surface of monocytes/macrophages, dendritic cells, and at relatively low levels on granulocytes. It is also expression on a small subset of NK cells. ILT1 acts as an activating receptor through its association with Fc receptor γ chain.
100tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: immunohistochemical staining of acetone-fixed frozen tissue sections, Costimulation and activation of myeloid cells skewing of dendritic cell differentiation
Application References(PubMed link indicates BioLegend citation): Lee DJ, et al. 2007 J. Immunol. 179 8128.
RRID: AB_2249947 (BioLegend Cat. No. 337904)
Structure: ILT/LIR family, Ig superfamily, 69 kD glycoprotein
Distribution: Monocytes/macrophages, dendritic cells, granulocytes (low), small subset of NK cells
Ligand/Receptor: Associate with Fc receptor γchain
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tedla N, et al. 2003. Proc. Natl. Acad. Sci. 100:1174 2. Nakajima H, et al. 1999. J. Immunol. 162:5
Gene ID: 11027
UniProt: View information about CD85h on UniProt.org
Clone: 24
Regulatory Status: RUO
Other Names: ILT1, LIR-7, LILRA2
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.