Biolegend, 338516, PE/Cyanine7 anti-human CD23 Antibody, 100tests

CD23 is a 45 kD protein, also known as Leu-20, FcεRII, IgE Fc receptor, BLAST-2, B6, and low affinity IgE receptor. It is a member of the Ig family, expressed on most mature B cells, B cells in follicular mantle (but not in proliferating germinal center cells, follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 responds to high levels of IgE by downregulating IgE secretion. In human monocytes, CD23 triggering results in release of pro-inflammatory cytokines including TNF-α, IL-1, IL-6, and GM-CSF. CD23 can be proteolytically cleaved to generate soluble CD23 fragments of various molecular weights. In chronic lymphocytic leukemia, levels of soluble CD23 in the serum can be used as a prognostic marker to identify patients at high risk for disease progression. Alternate splicing of exon 2 can also generate two cell-surface isoforms of CD23 differing by 6 amino acids in their cytoplasmic region.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Sugden B and Metzenberg S. 1983. J. Virol. 46:800-807.
Product Citations: Tran TM, et al. 2020. Immunity. 51(4):750-765. PubMed SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed Roerden M, et al. 2021. Cancers. 13(11):. PubMed
RRID: AB_2278308 (BioLegend Cat. No. 338516)
Structure: Ig family member, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells, monocytes, eosinophils, a subset of T cells, Langerhans cells
Function: Regulation of IgE synthesis in human monocytes; CD23 triggering results in release of pro-inflammatory cytokines including TNF-a, IL-1, IL-6, and GM-CSF
Ligand/Receptor: IgE, CD19/CD21/CD81
Cell Type: B cells, Dendritic cells, Eosinophils, Langerhans cells, Monocytes, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Ludin C, et al. 1987. EMBO J. 6:109. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038. 4. Armant M, et al. 1994. J. Exp. Med. 180:1005.
Gene ID: 2208
UniProt: View information about CD23 on UniProt.org
Clone: EBVCS-5
Regulatory Status: RUO
Other Names: Leu-20, FcεRII, IgE Fc Receptor, BLAST-2, B6, Low affinity IgE receptor
Isotype: Mouse IgG1, κ