Biolegend, 338712, PE/Cyanine7 anti-human CD85d (ILT4) Antibody, 100tests

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subset of ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while the inhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85d is a 95kD inhibitory receptor, also known as ILT4, LIR2, or MIR10. ILT4 is expressed on the surface of monocytes/macrophages, and dendritic cells. ILT4 acts as an inhibitory receptor through recruitment of SHP-1 and SHP-2 protein tyrosine phosphatases. The ligands of ILT4 are HLA-A, -B and nonclassical MHC-I molecule HLA-G1.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: negatively modulate myelomonocytic cells signaling. Enhance the binding of HLA-G tetramer to monocytes.
Application References(PubMed link indicates BioLegend citation): Colonna M, et al. 1998. J. Immunol. 160:3096 Allan DS. 1999. J. Exp. Med. 189:1149
RRID: AB_2750150 (BioLegend Cat. No. 338711) AB_2750151 (BioLegend Cat. No. 338712)
Structure: ILT/LIR family, Ig superfamily, 95kD
Distribution: Monocytes/macrophages, dendritic cells
Ligand/Receptor: MHC class I molecules (HLA-A, -B) and nonclassic class I molecule HLA-G1
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication Shiroishi M, et al. 2003. Proc. Natl. Acad. Sci. 100:8856 Colonna M, et al. 1998. J. Immunol. 160:3096 Lichterfeld M, et al. 2007. J. Exp. Med. 204:2813
Gene ID: 10288
UniProt: View information about CD85d on UniProt.org
Clone: 42D1
Regulatory Status: RUO
Other Names: CD85, ILT4, MIR10, Leukocyte Immunoglobulin-Like Receptor subfamily B member 2 (LILRB2), Leukocyte Immunoglobulin-like Receptor 2 (LIR-2)
Isotype: Rat IgG2a, κ