Biolegend, 339111, PerCP/Cyanine5.5 anti-human CD355 (CRTAM) Antibody, 25tests

CRTAM, (class-I MHC-restricted T-cell associated molecule), is homodimer protein belonging to Ig superfamily. It is expressed transiently on activated NK cells,NKT cells and a subset of CD8+ T cells. Nectin-like molecule-2 (Necl2, TSCL1) is the ligand of CRTAM. CRTAM plays an important role in cell adhesion and migration.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CRTAM-P815 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Kent S, et al. 2005. Blood. 106:779
RRID: AB_2783221 (BioLegend Cat. No. 339111) AB_2783222 (BioLegend Cat. No. 339112)
Structure: Dimer, Ig superfamily
Distribution: Activated NK cells and subset of CD8+ cells
Function: Adhesion and migration
Ligand/Receptor: Nectin-like molecule 2 (Necl2)
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules, MHC Antigens
Antigen References: 1. Arase N, et al. 2005. Intl. Immunol. 17:1227 2. Kennedy J, et al. 2000. J. Leukoc. Biol. 67:725
Gene ID: 56253
UniProt: View information about CD355 on UniProt.org
Clone: Cr24.1
Regulatory Status: RUO
Other Names: Class I-MHC-Restricted T-cell Associated Molecule
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.