Biolegend, 340604, PE anti-human CD300F (IREM-1) Antibody, 100tests

IREM-1 (immune receptor expressed by myeloid cell 1) is a type I single transmembrane protein encoded by human chromosome 17q24.25. A member of the CD300 (CMRF-35) family and the Ig superfamily, IREM-1 is also known as CD300F, or CD300LF (CD300-like member F). CD300F is expressed on monocytes, granulocytes, and myeloid leukemia cell lines. The cytoplasmic domain of IREM-1 contains an ITIM and associates with the P85α subunit of PI3K (phosphoinositide 3-kinase). IREM-1 also functions as an inhibitory receptor.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: Coengagement of IREM-1 with UP-D2 inhibits FcReI-mediated degranulation, immunoprecipitation
Application References(PubMed link indicates BioLegend citation): Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690 Lee SM, et al. 2011. J. Immunol. 186:6296. PubMed.
RRID: AB_2074121 (BioLegend Cat. No. 340604)
Structure: Type I single transmembrane protein with cytoplamic ITIM, associates with p85α of PI3K, Ig superfamily
Distribution: Monocytes, granulocytes, and myeloid leukemia cell lines
Function: Inhibition of myeloid function
Cell Type: Monocytes, Granulocytes, Leukemia
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Marquez JA, et al. 2007. J. Mol. Bio. 367:310 2. Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690 3. Alvarez-Errico D, et al. 2007. J. Immunol. 178:808
Gene ID: 146722
UniProt: View information about CD300F on UniProt.org
Clone: UP-D2
Regulatory Status: RUO
Other Names: CD300LF, IREM-1, IgSF13,
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.