Biolegend, 341436, Spark Blue™ 550 anti-human CD79b (Igβ) Antibody, 100tests

CD79 is a heterodimeric molecule comprised of an α-chain (CD79a) and β-chain (CD79b). A 37-39 kD type I integral membrane protein CD79b is non-covalently associated with CD79a and cell surface IgM to form the B-cell receptor (BCR) complex. CD79b is expressed on the surface of surface Ig (sIg)-positive B cells and in the cytoplasm of sIg-negative B cells. It is essential for signal transduction after surface Ig crosslinking.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Nakamura T, et al. 1992. P. Natl. Acad. Sci. USA 89:8522. Garcia Vela J, et al. 1999. Leukemia. 13:1501. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Rossi EA, et al. 2014. PLoS One. 9:98315. PubMed
RRID: AB_3106019 (BioLegend Cat. No. 341435) AB_3106019 (BioLegend Cat. No. 341436)
Structure: 37-39 kD type I integral membrane protein, associate with CD79a and cell surface IgM to form the B-cell receptor (BCR) complex, Ig superfamily.
Distribution: B cells
Function: From BCR complex, signal transduction
Cell Type: B cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc. 2. Dylke J, et al. 2007. Immunol. Lett. 112:47
Gene ID: 974
UniProt: View information about CD79b on UniProt.org
Clone: CB3-1
Regulatory Status: RUO
Workshop: VI CD79.1
Other Names: Igb, Igβ (Ig-beta), B29
Isotype: Mouse IgG1, κ