Biolegend, 341504, PE anti-human CCR10 Antibody, 100tests

CCR10, also known as GPR-2, is a G-protein-coupled seven transmembrane CC-chemokine receptor. It is the receptor of CCL27 (CTACK/ALP/ILC/ESkine) and CCL28 (MEC) and is expressed on a small subset of T memory cells, IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts. The interaction of CCR10 with its ligands plays a role in the regulation of T cell homing into cutaneous site and IgA-secreting cells migration.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: N-terminal peptide of human CCR10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: It has been observed that clone 6588-5 can interact with some tandem-dye antibody conjugates during multi-color staining, potentially leading to unwanted staining. These dyes include PE/Cyanine7, PE/Cyanine5, PE/Dazzle™ 594, APC/Cyanine7, APC/Fire™ 750, and PerCP/Cyanine5.5. This interaction can be resolved by sequentially staining with the 6588-5 antibody first and then followed by other antibodies of interest.
Product Citations: Liu RX, et al. 2023. iScience. 26:106140. PubMed Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed Sugita S, et al. 2013. Invest Ophthalmol Vis Sci . 54:6926. PubMed Qualai J, et al. 2016. PLoS One. 11: 0156605. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Kubin ME, et al. 2017. Acta Derm Venereol. 97:449. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Zamarin D, et al. 2020. Clin Cancer Res. 26:4531. PubMed
RRID: AB_1595542 (BioLegend Cat. No. 341503) AB_1595446 (BioLegend Cat. No. 341504)
Structure: G-protein-coupled seven transmembrane protein
Distribution: Small subsets of T memory cells and IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts
Function: T cell homing into cutaneous site, regulation of IgA-secreting cells migration
Ligand/Receptor: CCL27 (CTACK/ALP/ILC/ESkine), CCL28 (MEC)
Cell Type: B cells, Endothelial cells, Fibroblasts, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Hudak S, et al. 2002. J. Immunol. 169:1189 2. Kunkel EJ, et al. 2003. J. Clin. Invest. 111:1001 3. Homey B, et al. 2002. Nature Medicine. 8:157 4. Nakayama T, et al. 2002. J. Virol. 76:3072
Gene ID: 2826
UniProt: View information about CCR10 on UniProt.org
Clone: 6588-5
Regulatory Status: RUO
Other Names: GPR-2, C-C chemokine receptor 10
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.