Biolegend, 341605, FITC anti-human CX3CR1 Antibody, 25tests

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CX3CR1-EGFP fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Nishimura M, et al. 2002. J. Immunol. 168:6173. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
Product Citations: Healy LM, et al. 2020. Glia. 68:811. PubMed Benmeziane K, et al. 2022. STAR Protoc. 3:101815. PubMed Huuhtanen J, et al. 2023. J Clin Invest. 133: . PubMed Fadini G, et al. 2014. Atherosclerosis. 237:805. PubMed Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed Staumont-Sallé D, et al. 2014. J Exp Med. 211:1185. PubMed Feuser K, et al. 2012. Cytokine. 58:178. PubMed Cavarelli M, et al. 2022. iScience. 25:104346. PubMed Kim M, et al. 2017. Cytokine. 10.1016/j.cyto.2017.07.007. PubMed Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed Hasselmann J, et al. 2020. Neuron. 103(6):1016-1033. PubMed Zhao T, et al. 2012. PLoS One. 7:e43399. PubMed Scheiblich H, et al. 2021. Cell. 184:5089. PubMed Kleinnijenhuis J, et al. 2012. Proc Natl Acad Sci U S A. 109:17537. PubMed Berg P, et al. 2012. Clin Vaccine Immunol. 1.327777778. PubMed Remmerswaal E, et al. 2012. Blood. 119:1702. PubMed Lee CH, et al. 2018. Elife. 7:e32532. PubMed Verschoor C, et al. 2014. PLoS One. 9:104522. PubMed
RRID: AB_1626276 (BioLegend Cat. No. 341605) AB_1626272 (BioLegend Cat. No. 341606)
Structure: G-protein-coupled seven transmembrane receptor
Distribution: NK cells, T cell subset, monocytes/macrophages, dendritic cells
Function: cell adhesion and migration
Ligand/Receptor: CX3CL1 (Fractalkine, FKN, neurotactin)
Cell Type: Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595.
Gene ID: 1524
UniProt: View information about CX3CR1 on UniProt.org
Clone: 2A9-1
Regulatory Status: RUO
Other Names: V28, GPR13, Chemokine C-X3-C receptor 1
Isotype: Rat IgG2b, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.