Biolegend, 342919, PE/Dazzle™ 594 anti-human TCR Vα24-Jα18 (iNKT cell) Antibody, 25tests

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.
Application References(PubMed link indicates BioLegend citation): Rout N, et al. 2010. PLoS One 5:e9787. (FC)
RRID: AB_2566311 (BioLegend Cat. No. 342919) AB_2566312 (BioLegend Cat. No. 342920)
Structure: Invariant CDR3 region of TCR Vα24-JαQ
Distribution: Invariant NKT cells
Ligand/Receptor: Glycolipid loaded CD1d
Cell Type: NKT cells
Biology Area: Immunology
Molecular Family: TCRs
Antigen References: Thomas SY, et al. 2003. J. Immunol. 171:2571. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756. Montoya CJ, et al. 2007. Immunology. 122:1. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID: 28659
UniProt: View information about TCR Valpha24-Jalpha18 on UniProt.org
Clone: 6B11
Regulatory Status: RUO
Other Names: TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype: Mouse IgG1, κ