Biolegend, 343107, APC anti-human CD7 Antibody, 25tests

CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KG1a cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported (for the relevant formats) applications include proteogenomics2.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University Press. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed Minagawa A, et al. 2018. Cell Stem Cell. 1.548611111. PubMed Ishihara Y, et al. 2017. J Virol. 10.1128/JVI.00974-17. PubMed Lv L, et al. 2019. Exp Ther Med. 17:1360. PubMed Bi Y, et al. 2020. Cell Reports. 30(11):3917-3931. PubMed Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
RRID: AB_1877156 (BioLegend Cat. No. 343107) AB_2291325 (BioLegend Cat. No. 343108)
Structure: Ig superfamily, type I transmembrane glycoprotein, 40 kD
Distribution: T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets
Function: T cell activation
Ligand/Receptor: Cytoplasmic domain associates with P13-kinase
Cell Type: Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. 2. Stillwell R, et al. 2001. Immunol. Res. 24:31. 3. Rabinowich H, et al. 1994. J. Immunol. 152:517.
Gene ID: 924
UniProt: View information about CD7 on UniProt.org
Clone: CD7-6B7
Regulatory Status: RUO
Workshop: IV T-164
Other Names: gp40
Isotype: Mouse IgG2a, κ