Biolegend, 343503, FITC anti-human CD34 Antibody, 25tests

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
Application References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations: Bowley TY, et al. 2022. Cancer Res Commun. 2:1436. PubMed Filipello F, et al. 2023. Acta Neuropathol. 145:749. PubMed Ishiuchi N, et al. 2023. Stem Cell Res Ther. 14:121. PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Han X, et al. 2023. Stem Cells Int. 2023:8992284. PubMed Gan T, et al. 2021. Cell Rep. 37:109824. PubMed Benjamin DI, et al. 2022. Cell Metab. 34:902. PubMed Huang J, et al. 2022. Clin Exp Pharmacol Physiol. 49:881. PubMed Zhang T, et al. 2022. IUBMB Life. 74:1081. PubMed Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed Fu H, et al. 2023. Regen Ther. 22:7. PubMed Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed Chen X, et al. 2023. Exp Ther Med. 25:99. PubMed Wei X, et al. 2022. Mol Cell. 82:1225. PubMed Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed Casetti L, et al. 2013. Cancer Res. 73:2052. PubMed Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed Padmanabhan U, et al. 2020. Eur J Haematol. 104:170. PubMed Tancharoen W, et al. 2019. Mol Med Rep. 19:5123. PubMed Sung SE, et al. 2021. Int J Mol Sci. 22:. PubMed Sung SE, et al. 2021. Vet Sci. 8:. PubMed Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed Wu J, et al. 2021. Front Immunol. 12:601611. PubMed Sung SE, et al. 2021. Asian Spine J. Online ahead of print.. PubMed Kerr BA, et al. 2021. Experimental Cell Research. 399(2):112456. PubMed Santos J, et al. 2013. J Transl Med. 11:18. PubMed Sarkar TJ, et al. 2020. Nat Commun. 11:1545. PubMed Judson RN et al. 2018. Cell stem cell. 22(2):177-190 . PubMed Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Yan K, et al. 2022. iScience. 25:104822. PubMed Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed Zeng J, et al. 2020. Nat Med. 26:535. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed Fathy El-Metwaly N, et al. 2021. Asian Pac J Cancer Prev. 22:3237. PubMed Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed Busch A, et al. 2020. Int J Mol Sci. 21:. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed Matsuyama H, et al. 2019. Sci Rep. 9:13181. PubMed Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed Pauken CM, et al. 2021. Cancers (Basel). 13:. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
RRID: AB_1731923 (BioLegend Cat. No. 343503) AB_1731852 (BioLegend Cat. No. 343504)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selectin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 581
Regulatory Status: RUO
Workshop: V MA27
Other Names: Gp105-120, My10
Isotype: Mouse IgG1, κ