Biolegend, 343516, PE/Cyanine7 anti-human CD34 Antibody, 100tests

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
Application References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations: Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed Mishra P, et al. 2021. Indian J Hematol Blood Transfus. 37:391. PubMed Vanuytsel K, et al. 2022. Cytometry A. 101:903. PubMed Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed Aru B, et al. 2023. Balkan Med J. 40:117. PubMed Calvanese V, et al. 2022. Nature. 604:534. PubMed Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed Stavish D, et al. 2020. Nat Commun. 3.922916667. PubMed Laustsen A, et al. 2021. Elife. 10:. PubMed Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed Dyring-Andersen B, et al. 2020. Nat Commun. 4.338194444. PubMed Sun Z, et al. 2018. Oncotarget. 9:10945. PubMed Amoah A, et al. 2021. Haematologica. Online ahead of print. PubMed Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed Koide M, et al. 2018. Tohoku J Exp Med. 244:15:00. PubMed BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed Ng E, et al. 2016. Nat Biotechnol. 34:1168-1179. PubMed Hsieh W, et al. 2015. Gut. 64:312. PubMed Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed Gillissen MA, et al. 2018. Blood. 131:131. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Kraus H, et al. 2014. J Immunol. 192:1044. PubMed Perna F, et al. 2017. Cancer Cell. 32:506. PubMed Ochi K, et al. 2014. Stem Cells Trans Med. 3:792. PubMed Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed Li X, et al. 2021. Hemodial Int. 25:489. PubMed Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed Garciaz S, et al. 2019. Clin Epigenetics. 0.55625. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed Yoshioka S 2014. Sci Rep. 4:5066. PubMed
RRID: AB_1877252 (BioLegend Cat. No. 343515) AB_1877251 (BioLegend Cat. No. 343516)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selectin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 581
Regulatory Status: RUO
Workshop: V MA27
Other Names: Gp105-120, My10
Isotype: Mouse IgG1, κ