Biolegend, 343611, PerCP/Cyanine5.5 anti-human CD34 Antibody, 25tests

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.
Application References(PubMed link indicates BioLegend citation): Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations: Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed Lina IA, et al. 2022. Otolaryngol Head Neck Surg. 166:712. PubMed Bao EL, et al. 2020. Nature. 769:586. PubMed Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed Kiehlmeier S, et al. 2021. Leukemia. Online ahead of prin. PubMed Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
RRID: AB_2566787 (BioLegend Cat. No. 343611) AB_2566788 (BioLegend Cat. No. 343612)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selecin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 561
Regulatory Status: RUO
Other Names: Gp105-120, My10, Mucosialin
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.