Biolegend, 343908, PerCP/Cyanine5.5 anti-human CD166 Antibody, 100tests

CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Cultured human thymic epithelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1
Application References(PubMed link indicates BioLegend citation): Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC)
RRID: AB_2814265 (BioLegend Cat. No. 343907) AB_2814266 (BioLegend Cat. No. 343908)
Structure: A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.
Distribution: CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.
Function: Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
Ligand/Receptor: CD6
Cell Type: Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Aruffo A, et al. 1997. Immunol Today. 18(10):498 2. Patel DD, et al. 1995. J. Exp. Med. 181:2213 3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563 4. Horst D, et al. 2009. Cancer Invest. 22:1
Gene ID: 214
UniProt: View information about CD166 on UniProt.org
Clone: 3A6
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.